Hi all,

I am trying to figure out the logic of how trees are split, for example the method used by splitstree. I have read various papers and the splitstree manual, however to no avail. Using a simple tree like the following:

```
tree="((t5:2.161175,t6:0.161175):0.392293,((t4:0.104381,(t2:0.075411,t1:0.075411):1):0.065840,t3:0.170221):0.383247)"
```

Splitstree gives the following splits:

```
2.161175 Split t5,
0.161175 Split t5 t4 t2 t1 t3,
0.104381 Split t5 t6 t2 t1 t3,
0.075411 Split t5 t6 t4 t1 t3,
0.075411 Split t5 t6 t4 t2 t3,
1.0 Split t5 t6 t4 t3,
0.06584 Split t5 t6 t3,
0.170221 Split t5 t6 t4 t2 t1,
0.77554 Split t5 t6,
```

Could anyone please explain how this is carried out, in particular the T5, T6 distance. Thanks

Thanks for all your help. I have input the tree into R and I just want to ensure I have grasped your explanation correctly. The "aim" is to attach each tip to the rest of the tree? e.g. Attach T5 to the rest of the tree (T6, T4, T3, T2, T1) is 2.161175. and this is carried out for each tip. namely T6, T4, T3, T2, and finally T1. Resulting in the following: t5: 2.161175 t6: .161175 t4: .104381 t3: .170221 t2: .075411 t1: .075411

The distances to attach the sub trees is calculated: T1 T2 to the rest of the tree (T3, T4, T5, T6) : 1 T1,T2,T4 (T3,T5,T6): .065840

Am I correct in this thinking thus far?

Finally T5 T6: to attach this sub tree to the rest of the tree is .77554. However I am still unsure why it is the addition of R labels 1 and 4. To attach T5,T6 to T4,T3,T2,T1 why is it not just R label 4.

Thanks very much for your help, greatly appreciated.